Production Process of Sclerotium Gum
- Crude product preparation: the Sclerotium sclerotium fermentation broth is precipitated with ethanol, which is the crude Sclerotium sclerotium gum;
- Protein denaturation: dissolve the crude sclerotin gum into a solution and heat it;
- Activated carbon adsorption: adjust the pH of the solution in step (2) to 5.0-7.0, add activated carbon to adsorb for 0.5-2h, adjust the pH again to above 8.0 and filter to obtain the supernatant;
- Alcohol precipitation: adjust the pH of the supernatant of step (3) to 5.0-7.0, and add ethanol to obtain microsclerotia gum fibrous precipitate;
- Drying: The fibrous precipitate of Sclerotium sclerotium is dried in vacuum to obtain Sclerotium sclerotium powder.
- In the step (1) the viscosity of the fermentation broth of Sclerotium sclerotium ≥ 10000 mPa.s.
- In the step (2), the concentration of Sclerotium sclerotium in the solution is 0.25-1% (w/v); the heating temperature is 85-95° C., and the temperature is maintained for 10-30 min.
- In the step (3), the amount of activated carbon is 0.5-1.5% (w/v); the adsorption temperature is 55-70°C, and the time is 0.5-2h; the pH value is adjusted to 5.0-7.0 before adsorption, and the pH value is adjusted after adsorption Above 8.0.
- The pH value after adjustment in the step (4) is 5.0-7.0.
- In the steps (1) and (4), the ethanol concentration is 90-100% (v/v), and the amount of ethanol is 1.5-2 times the volume of the solution.
- In the step (5), the drying temperature is 60-65°C; the whiteness of the sclerotium powder is more than 80%, the viscosity of its 1% solution is 1500-2500 mPa.s, and the light transmittance is more than 90%.
The fermentation broth of Sclerotium in the step (1) is produced by the following steps:
- Strain activation: inoculate Sclerotium sclerotium in a slant medium, and cultivate for 2-3 days at a constant temperature.
- Preparation of seed culture solution: Rinse the cultivated strain slope with sterile water to obtain a Sclerotium sclerotium suspension; connect the suspension to the liquid culture medium and shake it, and then shake it in a constant temperature shaker for 2-3 days. Obtain seed culture solution.
- Fermentation broth preparation: inoculate the seed culture broth into the liquid culture medium, culture for 3-7 days under aeration and constant temperature stirring, control the pH value during the fermentation process, intermittently supplement sugar, and obtain the Sclerotium fermented broth at the end of the fermentation.
- The medium contains 20-50 g/L glucose, 1 g/L yeast extract, 2-4 g/L sodium nitrate, 0.5-2 g/L potassium dihydrogen phosphate, 0.3-1 g/L magnesium sulfate, pH The value is 4.0-5.0.
- The culture temperature is 28±2°C; the inoculum amount is 5-10% (v/v).
- The rotating speed of the shaker is 200 rpm.
- The volume of the 5L fermenter is 4L, the fermentation culture stirring speed is 200-300rpm, the aeration rate is 4L/min, and the pH value is 4.0-6.0.
A use of the Sclerotium produced by the above method in the preparation of anti-oxidant cosmetics and foods.
